產(chǎn)品英文名稱：Sonic Hedgehog, human，SHH-025
產(chǎn)品中文名稱：人SHh效應(yīng)蛋白

Sonic Hedgehog, human產(chǎn)品介紹：
Hedgehog基因zui早在果蠅中作為體節(jié)極性基因被發(fā)現(xiàn)，Hedgehog基因編碼一種分泌信號蛋白，突變會使無毛部分變成有毛部分，所以又戲稱為“刺猬"基因。哺乳動物發(fā)現(xiàn)其同源基因有3個(gè)，分別為Sonic Hedgehog（SHh）、Indian Hedgehog（IHh）和Desert Hedgehog（DHh）。SHh 前體蛋白要經(jīng)過一系列剪切與修飾，首先SHh 前體蛋白在內(nèi)質(zhì)網(wǎng)剪切去掉信號肽,，然后經(jīng)過自我酶解形成19 kDa N 端蛋白和25 kDaC 端蛋白，SHh-N 端蛋白發(fā)揮了SHh信號分子的功能，執(zhí)行SHh信號通路的主要功能。C 端蛋白擁有末端膽固醇轉(zhuǎn)移酶活性，可以將膽固醇分子轉(zhuǎn)移到SHh N 端蛋白的C 端，末端的膽固醇分子可以和細(xì)胞膜結(jié)合而錨定SHh N 端蛋白，從而有效控制SHh N 端蛋白的作用區(qū)域。

經(jīng)典的SHh信號通路是由SHh配體、2個(gè)跨膜蛋白質(zhì)收徒Patched（Ptch）和 Smoothened（Smo）組成的受體復(fù)合物，以及下游的轉(zhuǎn)錄因子Gli蛋白（Gli-1、Gli-2和Gli-3）組成。

Hedgehog信號在胚胎發(fā)生過程中細(xì)胞分化和器官形成起到非常重要的作用，在成體一些組織器官Hh信號通路仍然活躍，調(diào)節(jié)干細(xì)胞靜止與增殖，Hh基因在不同組織過度表達(dá)或靶基因突變等，均可導(dǎo)致細(xì)胞特異性增生，參與腫瘤的發(fā)生與發(fā)展。

? Sonic Hedgehog, human蛋白活性比從原核細(xì)胞純化得到的高10-15倍
? 生物活性在Swiss 3T3細(xì)胞通過Gli報(bào)告基因檢測，The EC50  3 to 10 ng/ml
? Sonic Hedgehog, human產(chǎn)品是通過哺乳動物細(xì)胞純化得到，并經(jīng)過SDS-PAGE 和HPLC雙重鑒定純度大于85%

Sonic Hedgehog, human產(chǎn)品英文簡述：
The hedgehog (Hh) gene encodes a secreted protein originally identified in Drosophila. The vertebrate homologs of Hh comprise of Sonic (SHh), Desert (DHh) and Indian (IHh). SHh plays an important role in embryonic development and in diseases such as cancer. Human SHh precursor is a 45kDa protein. An autocatalytic reaction yields a 19 kDa N-terminal domain that is biologically active. Because of unique post-translational modifications, SHh produced in mammalian cells is much more biologically active than SHh produced from E. coli and is devoid of artifact associated with the E. coli products commercially available from other manufacturers.

? Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis
? Biological Activity: The activity was determined by using a Gli reporter gene assay in Swiss 3T3 cells. The EC50 ranges from 3 to 10 ng/ml
? For Research Use Only. Not for Use in Humans